Video Review 1 – Glycolysis Part 2: Fates of Pyruvate
This video was taken from the BIOCHEMJM Youtube channel. First of all , I must say that this topic is my favourite topic throughout this course , this video goes into detail about what happens after the pyruvate is produced during glycolysis. Oddly enough looking through youtube , although a lot of videos regarding Glycolysis were found , videos detailing the use of pyruvate , or the fates of pyruvate were few and far in between , hence from my point of view I would regard this video as the best and most in depth one that I’ve seen concerning the topic so far. The video has three main points , 1) the use of pyruvate under anaerobic conditions to produce ethanol and carbon dioxide , 2) the use of pyruvate under anaerobic conditions to produce lactate and 3) the use of pyruvate under aerobic conditions to produce carbon dioxide and water. In the first point the video makes it goes into fermentation and lists the importance of fermentation , stating that it replenishes NAD+ for use in glycolysis. The videos goes into the reaction whereby , under anaerobic conditions , pyruvate is converted to acetaldehyde via the enzyme pyruvate decarboxylase and the use of the cofactors TPP and Mg2+ ions , this reaction produces carbon dioxide as a by product . The acetaldehyde is then converted to ethanol via the enzyme alcohol dehydrogenase , this reaction also converts NADH to NAD+. The second point made is the conversion of pyruvate to lactate under anaerobic conditions , the reaction occurs via the enzyme lactate dehydrogenase and in this reaction NADH is converted to NAD+. Lactate is produced in vigorously contracting muscles because in such circumstances there is an oxygen debt present and hence anaerobic conditions would be prevalent , and in erythrocytes because they lack mitochondria to produce energy otherwise. The third point discussed is the conversion of pyruvate to water and carbon dioxide under aerobic conditions. The first stage of this reaction is converting pyruvate to Acetyl-CoA , this reaction occurs via an enzyme complex referred to as the pyruvate dehydrogenase complex , which consists of three enzymes , this reaction produces carbon dioxide and uses CoA-SH as a cofactor , it was also noted in the video that in the reaction NAD+ is converted to NADH. The second stage of the conversion occurs via the citric acid cycle where the Acetyl-CoA is converted to carbon dioxide and water. Overall I must say that I enjoyed the video for a number of reasons ,such as , the length , the video is roughly about eighteen minutes long , this is GOOD because it allows a maximum of information to be absorbed in a smaller period of time , it also means that if one were to want to re watch the video , it wouldn’t seem like such a task , also what I have found is that because the download size of the video is relatively small and would not take up much memory , people are more inclined to put the video on their phone and watch in their free time to refresh themselves on the topic , this is what I personally do. Another reason I like this video is because after watching this video and attempting an online quiz I scored 94.5% , which is also GOOD , I think that the fates of pyruvate is an important topic since it explains why glycolysis even occurs to produce pyruvate in the first place. If I had to make a recommendation to better the video , I would include more dynamic slides or video clips , since the lecture is in a video format it allows moving images and flashy effects to be used , I think such inclusions would better help to keep the attention of the viewer.
In conclusion , the video is extremely informative , well produced , formatted nicely , it is interesting , flows one point into the other and is overall a great resource for anyone interested in glycolysis or biochemistry.